Lipase Immobilization on Silica Gel Using a Cross-linking Method

Abstract

The objective of this study was to develop a suitable method for immobilizing lipase. Although many investigators have studied lipase immobilization, detailed optimization of each step for lipase immobilization using cross-linking methods has been lacking. Therefore, we focused on establishing and optimizing each step of the immobilization procedure to improve procedural efficiencies. Lipase produced from Rhizopus oryzae was immobilized on silica gel using a cross-linking method. The immobilization procedures, such as the pretreatment of silica gel, silanization, crosslinking, glutaraldehyde modification, and elimination of unreated aldehyde groups, were all optimized. Furthermore, the stability of the immobilized lipase and its suitability for reuse were also evaluated.