Angewandte Chemie International Edition · 2016 · 256 citations · 27 references
Natural SciencesBiochemistryBiophysicsBiomedical ImagingBiophotonicsCytoskeletonCellular ImagingFluorescence ImagingFluorescence MicroscopyFluorescence SpectroscopyLight MicroscopySuper-resolution MicroscopyFluorescent Dye LabelingFluorescent DyesFluorophore DesignNatural DyesStimulated EmissionStimulated Emission Depletion MicroscopySuper-resolution Fluorescence ImagingSuper-resolution Fluorescence Microscopy
A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.
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A general method to improve fluorophores for live-cell and single-molecule microscopy
Jonathan B. Grimm, Brian P. English, Jiji Chen et al. · Nature Methods · 2015 · 1.6K citations · Full text
Fluorogenic probes for live-cell imaging of the cytoskeleton
Gražvydas Lukinavičius, Luc Reymond, Elisa D’Este et al. · Nature Methods · 2014 · 888 citations · Full text