Nucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nitrogen circuit of Neurospora crassa

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.8K citations · Full text

Genetics, Bacteriology, Bacteriophage +18

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Peter Rigby, Marianne Dieckmann, Carl Rhodes et al. · Journal of Molecular Biology · 1977 · 11.7K citations

Nucleic Acid Chemistry, High Specific Activity, Biochemistry +8

Rapid and efficient site-specific mutagenesis without phenotypic selection.

Thomas A. Kunkel · Proceedings of the National Academy of Sciences · 1985 · 7.3K citations · Full text

Engineering, Reverse Genetics, Efficient Site-specific Mutagenesis +14

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

Haim Aviv, Philip Leder · Proceedings of the National Academy of Sciences · 1972 · 6.9K citations

Glycobiology, Molecular Biology, Specific Mrnas +17