PubMed · 2000 · 10 citations · 0 references
Veterinary MedicineImmunologyMicrobiologyImmunizationImmunology Of Infectious DiseasesVaccinologyDna VaccinesNucleic Acid VaccinesSubunit VaccinesVaccinationVaccine Antigen DiscoveryVaccine-preventable DiseasesBacterial Vaccine AntigensBacterial VaccinesDeoxyribonucleic Acid (Dna) VaccineMycobacterium Tuberculosis InfectionPlasmid Vaccine VectorsTherapeutic Subunit VaccinesTuberculosis Vaccine DevelopmentVaccine Candidate Antigens
We have investigated the effect of subunit vaccines against infection with Mycobacterium leprae, employing DNA plasmids as the vaccine vectors, and the immunodominant 35 kDa protein of M. leprae as the candidate antigen. A DNA vaccine that expresses the M. leprae 35 kDa protein both stimulated interferon-gamma (IFN gamma)-secreting T cells in mice, and demonstrated protection against M. leprae-infection of mice.