Maturation effects on the NMR microimaging characteristics of single neurons

Abstract

We have used an isolated neuronal preparation of single Aplysia californica L7 cells to study the effects of development on the nuclear magnetic resonance (NMR) characteristics, T2 spin-spin relaxation rate (RT2) and apparent diffusion coefficient (D), of intracellular water within the nuclear and cytoplasmic compartments. These studies demonstrate a significant correlation of animal weight, but not age, with RT2. On the other hand, D was not significantly different as a function of either age or weight in single L7 neurons. Demonstration of maturation dependence in single cells is important in understanding the cellular origins of developmental effects on NMR characteristics in cell assemblies such as brain.