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Genotyping of alcohol dehydrogenase at the ADH2 and ADH3 loci by using a polymerase chain reaction and restriction-fragment-length polymorphism in Chinese alcoholic cirrhotics and non-alcoholics.
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1994
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GeneticsGenetic EpidemiologyHuman PolymorphismMolecular BiologyPathologyMolecular GeneticsGenomicsRestriction-fragment-length PolymorphismImmune-related Gene PolymorphismGenetic AnalysisGenotype-phenotype AssociationPolymorphic Adh2Public HealthMolecular DiagnosticsAlcohol DehydrogenasesAldehyde DehydrogenaseAutoimmune DiseaseBiochemistryLiver PhysiologyGenetic FactorGenetic VariationAllelic VariantGenetic DeterminantHepatologyHepatitisAlcohol DehydrogenaseChinese Alcoholic CirrhoticsMedicineChinese Population
We investigated the genotype of ADH2 and ADH3 in Chinese alcoholic cirrhotics and non-alcoholics by using a polymerase chain reaction and restriction-fragment-length polymorphism. This method is non-radioactive, easy to implement with good reproducibility. In the Chinese population, the frequencies of the ADH2*1 and ADH3*2 alleles were significantly higher in the alcoholic cirrhotic patients (53%; 23%) than in the viral hepatitis cirrhotics (32%; 8%) and the gastric and/or duodenal ulcer control patients (25%; 6%). On the other hand, the gastric and/or duodenal ulcer control patients and the viral hepatitis cirrhotic patients showed similar allele frequencies for the polymorphic ADH2 and ADH3 genes. These findings suggest that the alleles ADH2*2 and ADH3*1, coding for the high-Vmax beta 2-ADH and gamma 1-ADH, respectively, may play a protective role against alcoholism in Chinese patients.