The linkage of glyoxylase-I to the major histocompatibility complex in the rat.

Abstract

Abstract The enzymatic activity of glyoxalase I in lysates of rat red blood cells varied among 26 inbred strains: 23 had a high level of activity, three had a low level, and there was no sex difference within a strain. Studies in two backcross populations derived from parents with high and low levels of glyoxalase I activity, respectively, showed that the gene controlling this activity (Glo-1) was linked to the major histocompatibility complex at a distance of 4.6 (2.2 to 8.5) cM from RT1.A. It has two alleles, Glo-lh and Glo-11, which control high and low levels of enzymatic activity, respectively. The sex of the F1 hybrid parent did not influence the recombination frequency, and there was no selection against sex in the offspring. The glyoxalase I activity of the congenic line BN.1U(WF) was the same as that of the MHC donor strain; hence, the Glo-1 locus was carried as a passenger locus during derivation of this congenic line.