DEVELOPMENT OF GENETIC LINKAGE MAP OF LEAF HAIRINESS IN GOSSYPIUM HIRSUTUM (COTTON) USING MOLECULAR MARKERS

Abstract

Cotton (Gossypium hirsutum L.) genotype Rajhans (pilose) was crossed with FH-634 (glabrous) and developed F2 and F3 segregating populations. PCR based techniques RAPD and SSR were used to determine DNA markers linked with the trait of hairiness using 400 RAPD and 54 SSR primers. Prior to analysis, PCR conditions were optimized. Eight RAPD primer markers OPD19640, OPF-11630, OPG-06980, OPG-17500, OPH-131100, OPN-14890, OPO-11920, OPO-141200 were consistently detected in duplicate reactions. One SSR primer pair, JESPR-154 amplified a 150 bp DNA fragment in hairiness population. Polymorphic DNA markers were used to develop linkage map. Construction of genetic linkage map using RAPD and SSR markers revealed that the inheritance for leaf hairiness exists, which can be used to improve insect resistance in cotton.