Screening of Biological Activities (Antioxidant, Antibacterial and Antitumor) of Nerium oleander Leaf and Flower Extracts

Abstract

Oleander ( Nerium oleander L. Apocyanaceae) is an evergreen shrub, widely use for medicinal purposes. The objective of this work is to investigate the antioxidant, antibacterial and cytotoxic effects of the plant leaves (L) and flowers (F) extracts. These activities had been evaluated by DPPH, agar disc diffusion, agar dilution and MTT, respectively. Our results showed that methanol extracts (L & F) have high antioxidant activity with RC 50 value of 0.27, 0.2mg/mL respectively, dichloromethane (DCM) and methanol extracts (L & F) showed strong antibacterial activity against both gram negative and gram positive bacteria. MTT assay exhibited that dichloromethane extracts (L & F) have high cytotoxic effects against T 47 D, HepG-2 and K562 cell lines with IC50 value of 57.77, 233.42µg/mL, 55.90, 108.31µg/mL, 70.03, 102.31µg/mL, respectively.