Effect of growth hormones on micropropagation of Vitis vinifera L. cv. Perlette

Abstract

In vitro propagation of Vitis offers opportunities for increasing plant material for cultivation. Cultures were established and maintained in vitro on MS medium supplemented with BA (0, 5 and 10 µM) for shoot and NAA (0, 1, 5 and 10 µM) for callus induction. The best explant sterilization was achieved with 10% chlorox treatment either for 10 or 15 minutes. Oxidative browning was effectively controlled by three subcultures of explants on fresh media. Better shooting was observed on MS medium with 5 µM BA. Shoot proliferation (80%) was obtained by subculturing the micro cuttings on same media as used for shoot formation. Maximum rooting (80%) occurred on medium with 10 µM IBA. The highest callus induction was 80% from stem segments on 5 µM BA followed by leaf disk explants on NAA (1 µM). All callus cultures initiated aerial roots. Leaf disk explants (60%) developed embryoids on MS medium supplemented with NAA (2 mg/l).