Biobased approach for the synthesis, characterization, optimization and application of silica nanoparticles by fungus Fusarium oxysporum

Abstract

Objective: Nanoparticles, a building block of nanotechnology offers a broad application in several areas of science. Among various methods for nanoparticle synthesis, the utilization of microbes is one of the solutions for bioanalysis of biocomponents. Microbes which are non-pathogenic to humans are employed to reduce the metal ions. The objective of the study was to synthesize of inorganic meterials on nano and micro length scale. Methods: In this work, Fusarium oxysporum from wilted tomato plant was selected and silica nanoparticles were synthesized. The silica nanoparticles synthesis characterization was done using UV-spec., FTIR and SEM analysis. The appropriate conditions for silica nanoparticles synthesis optimization and the separation of nanoparticle biosynthetic protein were carried out. Then the efficiency of nanoparticle in DNA protection from enzymatic cleavages and dye decolourization also determined. Results: The studies showed the confirmation of isolated F. oxysporum and the synthesized silica nanoparticles surface plasmon band occurs at 280 nm. In FTIR analysis, the presence of a resonance at ca.1113 cm -1 and absorption bands at ca.1623.62 indicated the amide band in the particle. Conclusions: Thus, fungus mediated silica nanoparticles synthesis could be used in many biological researches.