The International Journal of Artificial Organs · 2004 · 55 citations · 35 references
EngineeringBioengineeringBiomedical EngineeringNeural EngineeringRegenerative MedicineTissue EngineeringExtracellular MatrixNerve RegenerationNerve RepairNeural RegenerationRegenerative ScaffoldsTissue Engineering ScaffoldsEngineered Nerve ConduitsNerve Gap BridgingNerve Guidance ConduitsNerve Regeneration BiomaterialsNerve Regeneration ScaffoldsNerve Tissue EngineeringPeripheral Nerve RegenerationPeripheral Nerve Regeneration Scaffolds
The goal of this study was to investigate if a three dimensional matrix, loaded homogeneously with Schwann cells and the neurotrophic factor LIF (leukemia inhibitory factor), enhances regeneration in a biodegradable nerve guidance channel as compared to non-structured cell suspensions. Therefore a 10 mm nerve gap in the buccal branch of the rat's facial nerve was bridged with tubular PCL (poly-epsilon-caprolactone) conduits filled with no matrix, Schwann cells, the three dimensional fibrin/Schwann cell matrix or the fibrin/Schwann cell matrix added with LIF Four weeks after the nerve defects were bridged histological and morphometric analyses of the implants were performed. In conclusion, the three dimensional fibrin/Schwann cells matrix enhanced the quantity and the quality of peripheral nerve regeneration through PCL conduits. The application of LIF prevented hyperneurotization. Therefore, tissue engineered fibrin/Schwann cells matrices are new invented biocompatible and biodegradable devices for enhancing peripheral nerve regeneration as compared to non-structured cell suspensions without neurotrophic factors.
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Use of an Aqueous Soluble Tetrazolium/Formazan Assay for Cell Growth Assays in Culture
Ann H. Cory, Terence C. Owen, J. A. Barltrop et al. · Cancer Communications · 1991 · 1.5K citations