2,3-diphosphoglycerate in red cells stored in acid-citrate-dextrose and citrate-phosphate-dextrose: implications regarding delivery of oxygen.

Abstract

Abstract Blood from 6 normal volunteer donors was stored for 3 weeks in conventional acid-citrate-dextrose (ACD) and in citrate-phosphate-dextrose (CPD). Determination of the concentration of 2,3,-diphosphoglycerate (DPG) and of P 50 values, the pO 2 at which 50 per cent saturation of hemoglobin with oxygen occurs, were made repeatedly during the storage period. In all samples the P 50 was higher, as was the concentration of DPG, in the blood samples stored in CPD. The differences were of such magnitude that insofar as the in vitro measurement of these parameters is concerned, blood stored in CPD for 2 to 3 weeks is more nearly physiological than the same blood stored in ACD.