Tissue Engineering Part A · 2015 · 58 citations · 33 references
MedicineBioengineeringBiomedical EngineeringCell DifferentiationGene ExpressionStem Cell BiologyBone DevelopmentBone FormationBone RegenerationBone TissueBone Tissue EngineeringOsteogenesisGene Expression SignaturesMarker Gene ExpressionMicroarray AnalysisMicroenvironmental Regulation Of OsteogenesisOsteoblast Gene ExpressionOsteogenic DifferentiationOsteogenic MarkersOsteogenic Signaling Pathways
In this study, human mesenchymal stem cells (hMSCs) were cultured on the hydroxyapatite (HA) and mineralized collagen (MC), and their proliferation, adhesion, and differentiation, especially the molecular mechanisms on gene level, were investigated. Proliferation and morphological responses of hMSCs and their osteogenic differentiation were detected by quantitative detection of alkaline phosphatase. Gene expression profilings were examined by microarrays, and the gene expression data were studied through gene ontology terms and pathway analyses. The results showed that MC promoted cell proliferation and osteogenic differentiation of hMSCs. Microarray analysis showed that MC was conducive to express osteogenesis-related genes, such as BMP-2, COL1A1, and CTSK, and stimulate osteogenic differentiation, such as osteoblast differentiation pathway and skeletal system development pathway.
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Gene Ontology: tool for the unification of biology
Michael Ashburner, Catherine A. Ball, Judith A. Blake et al. · Nature Genetics · 2000 · 44.6K citations · Full text
KEGG: Kyoto Encyclopedia of Genes and Genomes
Hiroyuki Ogata, Susumu Goto, Kazushige Sato et al. · Nucleic Acids Research · 1999 · 32.4K citations · Full text