Biochemical Journal · 1966 · 12 citations · 6 references
Life SciencesBiochemistryDevelopmental BiologyEmbryogenesisEmbryologyNeuroendocrinologyBlastocyst DevelopmentEmbryo ImplantationHypothalamic-pituitary RegulationRabbit PhysiologyRabbit ReproductionReproductive NeuroendocrinologyBirth Process In RabbitsBlastocyst FormationBlastocyst Transfer TimingEmbryonic ImplantationHypothalamic NeurosecretionHypothalamic Regulation Of ReproductionImplantation PhysiologyMetabolism In Preimplantation Development
1. Hypothalami from pregnant and non-pregnant rabbits were homogenized in sucrose and separated into fractions by differential and density-gradient centrifugation. 2. The fractions were examined for enzymes capable of inactivating oxytocin. 3. Enzyme activity was detected in the supernatant and one particulate fraction of both pregnant and non-pregnant animals. 4. The specific activity of the enzymes increased during early pregnancy, and coincided with the time of blastocyst spacing and implantation in the uterus.
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