Laboratory Application of the Dextran-Coated-Charcoal Radioimmunoassay of Insulin

Abstract

Abstract A modification of the method for radioimmunoassay of insulin is described, which achieves satisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution formula, modified for our conditions, proves to be a reliable substitute for the standard curve.