Chemical composition and antimicrobial activity of volatile constituents from the roots, leaves, and seeds of Arctium lappa L. (Asteraceae) grown in Egypt

Abstract

Background and objectiveAs no literature was traced dealing with the volatile constituents of the leaves or the seeds of Arctium lappa L., it was deemed of interest to carry out a gas chromatography/mass spectrometry (GC/MS) analysis and antimicrobial activity study of the volatile constituents of roots, leaves, and seeds of the plant grown in Egypt.Materials and methodsThe volatile constituents of the roots, leaves, and seeds were analyzed by GC/MS. The antimicrobial activity was tested using the agar well diffusion technique.Results and conclusionGC/MS of the volatile constituents from the leaves showed 19 identified compounds, the major being caryophyllene oxide (54.2%), followed by β-elemene (6.2%) and β-costol (4.0%). Analysis of the volatile constituents of the roots revealed 14 identified compounds, the major being caryophyllene oxide (51%), followed by aromadendrene (16%) and isoaromadendrene epoxide (6.4%). Analysis of the volatile constituents of the seeds revealed 22 identified compounds, the major being E-citral (28.8%), followed by geraniol (20.3%) and Z-citral (9.5%). The volatile constituents of the leaves and roots exhibited moderate antimicrobial activity against bacteria and significant antifungal activity, in comparison with the standards used, whereas the volatile constituents of the seeds showed moderate antimicrobial activity against bacteria and fungi.