International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine · 1979 · 24 citations · 15 references
Life SciencesGeneticsMolecular BiologyDna RepairRadiation BiophysicsRadiobiologyBiological Effects Of RadiationCellular Radiation ResponseDna DamageDna Strand BreaksRadiation BiologyTissue Responses To RadiationAlkali TreatmentDna Damage InductionDna Double-strand BreaksDouble-strand Dna BreaksGamma Irradiation EffectsIonizing Radiation-induced Dna BreaksRadiation-induced Dna DamageRepair Of Ionizing Radiation-induced Breaks
Rats, 5, 10 or 25 days old, were 60 Co gamma irradiated. The induction of DNA strand breaks was studied after killing the rats within 1 min after irradiation, and the repair of the induced breaks after various intervals up to 180 min. Cell suspensions were prepared from the brain and samples were transferred into alkaline solutions. The fraction of DNA remaining double-stranded after 30 min alkali treatment was estimated after separation of single- and double-stranded DNA on hydroxylapatite. The amount of DNA strand breaks induced per Gray (1--8 Gray) was found to be in accordance with earlier in vivo studies of the mouse small intestine and mouse spleen. The DNA strand breaks in the rat brain induced by 4 Gray 60Co gamma irradiation were repaired 30 min after irradiation in all age groups studied.
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Introduction to radiation chemistry
Environment International · 1991 · 1.7K citations
Age-Associated Changes in the DNA of Mouse Tissue
Gerald B. Price, Sohan P. Modak, Takashi Makinodan · Science · 1971 · 202 citations