JNCI Journal of the National Cancer Institute · 1976 · 27 citations · 0 references
Life SciencesBiochemistryPharmacologyHormone BiochemistryReproductive ToxicologySteroid BiochemistryHormone Binding ProteinsHormone ReceptorsHormone SignalingSteroid Hormone SignalingSteroid PharmacologyCompetitive Binding AssaysCompetitive Binding StudiesEstradiol AssayEstrogen MetabolitesEstrogen ReceptorsHormone Receptor Binding ProteinsMetabolite-protein BindingSucrose Gradient Centrifugation
The estrogen receptor binding properties of 3,9-dihydroxybenz[a]anthracene (3,9-diOHBA) were determined in uterine cytosol of immature Sprague-Dawley rats by competitive binding experiments with [3H]estradiol and sucrose density centrifugation. 3,9-DiOHBA inhibited estradiol binding to the 8S binding protein at a concentration (1.2 X 10(-5) M) approximately equal to that of nafoxidine-HCl (3 X 10(-5) M) required to inhibit estradiol-specific binding, and bioassay for estrogenic activity substantiated this finding.