International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine · 1964 · 13 citations · 20 references
Life SciencesBiochemistryMolecular BiologyNucleic Acid BiochemistryRadiation BiophysicsRadiobiologyBiological Effects Of RadiationCellular Radiation ResponseDna DamageLiver RegenerationRadiation BiologyRadiation PhysiologyActivitiesCytological FeaturesDna Damage InductionEnzyme ActivityPhysiological Effects Of IrradiationRadiation-induced Dna DamageRadiation-induced Genetic EffectsRadiobiological Response
SummaryFree and specific activities of acid DNAase have been measured in the thymus and regenerating liver of rats after 700 r whole-body x-irradiation. Labilization of DNA has been evaluated in the same organs by the amount of DNA extracted in solution of increasing concentrations of sodium trichloracetate.The increase in DNAase specific activity observed in irradiated thymus is associated with increasing lability of the DNA-protein bonds.In normal regenerating liver, the specific activity of acid DNAase also increases, and this rise of enzyme activity is paralleled by an increased labilization of DNA.Irradiation of regenerating liver does not result in a further increase of acid DNAase specific activity, nor in a greater lability of DNA. Possible correlations between these changes and the cytological alterations after irradiation are discussed.
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