Parallel assessment of Th17 cell frequencies by surface marker co‐expression versus ex vivo <scp>IL</scp>‐17 production in <scp>HIV</scp>‐1 infection

Abstract

The definition of Th17 cells by surface markers might overestimate their frequency in comparison to functional assessment of IL-17 production by ICS, regardless of the HIV infection status. However, both methods yield proportionate results with reduced absolute numbers of Th17 cells in untreated HIV disease, reflecting the depletion of total CD4+ T cells in viremic HIV patients, and restoration with cART. © 2016 International Clinical Cytometry Society.