Sellimonas intestinalis gen. nov., sp. nov., isolated from human faeces

Abstract

A Gram-stain-positive and obligately anaerobic bacterial strain, BR72^T, forming ivory yellow colonies was isolated from a faecal sample of a healthy Korean woman. 16S rRNA gene sequence analysis indicated that strain BR72^T belongs to <i>Clostridium</i> cluster XIVa and represents a distinct phyletic line within the family <i>Lachnospiraceae</i>. The most closely related strains were <i>Clostridium nexile</i> DSM 1787^T (94.1 % 16S rRNA gene sequence similarity), <i>Coprococcus comes</i> ATCC 27758^T (93.5 %), <i>Ruminococcus torques</i> ATCC 27756^T (93.5 %), <i>Ruminococcus lactaris</i> ATCC 29176^T (93.5 %), <i>Clostridium aerotolerans</i> DSM 5434^T (93.1 %) and <i>Eubacterium fissicatena</i> DSM 3598^T (92.9 %). The DNA G+C content of strain BR72^T based on its genome sequence was 45.3 mol%. The major cellular fatty acids were C_{16 : 0}, C_{14 : 0}, and iso-C_{17 : 1} I and/or anteiso-C_{17 : 1} B. Acetic acid was produced from glucose fermentation. Other physiological and biochemical comparisons allowed the phenotypic differentiation of strain BR72^T from the members of the family <i>Lachnospiraceae</i>. Based on the phylogenetic and phenotypic findings, this strain is considered to represent a novel species of a new genus belonging to the family <i>Lachnospiraceae</i> and the name <i>Sellimonas intestinalis</i> gen. nov., sp. nov. is proposed. The type strain of <i>Sellimonas intestinalis</i> is BR72^T ( = KCTC 15479^T = JCM 30749^T).