The Synergistic Effects of Shear Stress and Cyclic Hydrostatic Pressure Modulate Chondrogenic Induction of Human Mesenchymal Stem Cells

Abstract

Differentiation of ASCs was shown by a modest increase in gene expression profiles. In general, BSCs expressed higher levels of chondrogenic gene expression than ASCs after 3 weeks. A greater effect on Col II and SOX9 mRNA expression was observed when combined loadings were applied. Results may be applied in determining the proper loading sequence for obtaining functional target cells in cartilage engineering applications.