Photomedicine and Laser Surgery · 2015 · 48 citations · 19 references
Natural SciencesBiophysicsCell BiologyBiophotonicsRadiation BiophysicsStem Cell BiologyAnimal Cell CultureBiomedical Laser ApplicationsCellular Radiation ResponseLaser-tissue InteractionRadiation Effects On Stem CellsStem Cell ProliferationBone Marrow Mesenchymal Stem CellsFree Radical GenerationLaser Energy ParametersLaser Irradiation ParametersLow-dose Radiation Effects On Stem CellsPeroxidation ProcessesReactive Oxygen Species GenerationStem Cell Proliferation After Irradiation
OBJECTIVE: The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells. BACKGROUND DATA: Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation. METHODS: Low-level laser (wavelength, 660 nm; power output, 50 mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production). RESULTS: The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12 J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8 J/cm(2). CONCLUSIONS: The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
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Biphasic Dose Response in Low Level Light Therapy
Ying‐Ying Huang, Aaron Chen, James D. Carroll et al. · Dose-Response · 2009 · 1.1K citations · Full text