Antibacterial properties of new calcium based cement prepared from egg shell

Abstract

Aims: The study was conducted to evaluate the pH and antimicrobial properties of newly prepared calcium based cement, polycarboxylate cement and biodentine material. Methods: The new cement was prepared from egg shell, for pH measurements, 9 standard discs (6 mm in diameter and 2 mm in thickness) were prepared for each material, The antibacterial effects of set specimens against Streptococcus mutans, oral Lactobacillus and Enterococcus faecalis were assessed by agar diffusion tests, 90 discs shaped specimens (30 of each type of materials; 6 mm in diameter x 2 mm in thickness) were prepared. Three discs, one for each material was placed on each agar plate, and the plates incubated for 24 hours. After incubation, the diameter of inhibition zone was measured at three different points and the mean value was used as the result of the specimen. Results: Statistically significant differences were identified between cements, calcium based cement and biodentine exhibited higher mean values of pH than polycarboxylate cement, ANOVA and Duncan's multiple range test were done to evaluate the effect of materials against each type of bacteria, it showed that the inhibition zones produced by experimental cement were statistically significantly larger than those produced by the other materials. Conclusion: Within the limitation of this research, it was concluded that new calcium based cement has a better antimicrobial properties than biodentine and polycarboxylate cement.