High frequency of shoot regeneration on leaf explants of Bacopa monnieri

Abstract

Plants of Bacopa monnieri were regenerated from entire leaf explants on Murashige and Skoog’s basal medium supplemented with different concentrations (2 – 12 µM) of cytokinin 6-benzylaminopurine. The largest numbers of adventitious shoot buds were induced in 100% cultures from the explant when 6-benzylaminopurine was used at a concentration of 6 µM. The rate of adventitious shoot bud regeneration was affected by the type of wounding and placement of the explant on the medium. The square leaf lamina produced the maximum number of shoots within eight weeks. Rooting was achieved in microshoots on ½ strength basal liquid medium supplemented with sucrose (1%) and indole-3-butyric acid (2 µM). The obtained plantlets could be successfully acclimatized ex vitro.