Concepedia

Isolation of Neospora caninum From the Brain of a Pregnant Sheep

T. Koyama, Yoshiyasu KOBAYASHI, Y. Omata, Michio Yamada, Hidefumi Furuoka, Ryuichiro Maeda, T. Matsui, Atsushi Saito, T. Mikami

Journal of Parasitology · 2001 · 52 citations · 17 references

DOI

Concepts

Abstract

Neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. The ewe showed no clinical signs. Tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum antibodies and by detecting N. caninum-specific DNA by polymerase chain reaction.

References

17

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Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction

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Rapid communication

Milton Μ. McAllister, J. P. Dubey, David S. Lindsay et al. · International Journal for Parasitology · 1998 · 735 citations

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Confirmation that the dog is a definitive host for Neospora caninum

David S. Lindsay, J. P. Dubey, Robert B. Duncan · Veterinary Parasitology · 1999 · 324 citations

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Neospora caninum: Specific Oligonucleotide Primers for the Detection of Brain "Cyst" DNA of Experimentally Infected Nude Mice by the Polymerase Chain Reaction (PCR)

Mat Yamage, Olivier Flechtner, Bruno Gottstein · Journal of Parasitology · 1996 · 264 citations

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