Isolation, Cloning and Expression of Mature Staphylokinase from Lysogenic Staphylococcus aureus Collected from a Local Wound Sample in a Salt Inducible E.coli Expression Host

Abstract

Staphylokinase, a 163 amino aci d bacterial protein from Staphylococcus aureus is reported to have a therapeutic function and used as a potential alternative for the available clot dissolving drugs and some countries proved to be a potent alternative over the available clot dissolving drugs. In the present study, sak gene is isolated from a lysogenically converted S.aureus and expressed in non-pathogenic E.coli expression host. The sak gene is cloned into pET-28a vector and expressed in an IPTG and salt induced E. coli strain (GJ1158). The mature recombinant sak protein expressed in E.coli is also further extracted and analyzed by various methods to demonstrate thrombolytic activity.