Isolation and Characterization of Respiratory Tract Bacterial Species from Domestic Animals with Pneumonic Lungs from Elphora Abattoir, Ethiopia

Abstract

For this study pneumonic lung tissue and tracheal swabs were collected from sheep, goat and cattle slaughtered at Elphora abattoir. From the total of 170 bacterial isolates from sheep lung tissue, 10.6% Bacillus species, 14.7% Mannheimia haemolytica and 42.4% Staphylococcus species were isolated aerobically. The tracheal cultures also indicated that 5.8% were Enterobacter species, 13% were Bibersteinia trehalosi and 66.7% were identified as M. haemolytica from the total of 69 aerobic cultures. Out of 192 bacteria isolated from aerobic inoculation of goat lung; 6.3% were M. haemolytica species, Micrococcus species 12.5%, Bacillus species, 19.8% and Staphylococcus 45.3%. The tracheal culture of goat showed Bacillus species 26.5%, M. haemolytica 27.7% and Enterobacter species 30.1%. Eight M. haemolytica species were among 93 bacterial isolates of aerobic cultures of cattle lung followed by Bacillus species, 17 and Staphylococcus species, 29. While M. haemolytica, B. trehalosi and Staphylococcus accounted for 8, 14 and 18 out of the total of 57 tracheal cultures respectively. A total of 522 bacterial isolates were detected via anaerobic culture, in which 6.2%% of the 131 isolates from sheep lung were Bacillus species, 25.4% were M. haemolytica and 40% were Staphylococcus species while B. trehalosi and Micrococcus species took 5.4% each from the total isolates. Whereas, 12.7% Enterobacter, 19.7% B. trehalosi and 60.6% M. haemolytica species were isolated from a total of 71 sheep trachea culture isolates. Among anaerobic cultures of goat lung tissue M. haemolytica species accounts for 11.2% followed by Bacillus species, 25.6% and Staphylococcus 36% out of the total 125 bacterial species. In the tracheal culture Enterobacter counts for 7 followed by Bacillus species, 14 and M. haemolytica species, 33 from 61 isolates. 7.8% B. trehalosi, 23.4% Bacillus species and 42.9% Staphylococcus were isolated species from the lung of the cattle, while from the trachea 25.0% M. haemolytica and Staphylococcus each were isolated anaerobically.