Standardization of a Suitable Protocol for in Vitro Clonal Propagation of Acorus calamus L. an Important Medicinal Plant in Bangladesh

Abstract

2 Abstract: The present investigation was carried out with a view to standardize an in vitro culture technique for mass propagation of important medicinal plant species, Acorus calamus L. Naturally grown rhizome tip was used as explants for initial culture. The explants were cultured on standard MS (Murashige and Skoog, 1962) medium supplemented with different concentration and combination of cytokinines and auxines for primary shoot proliferation. The best shoot proliferation was observed in MS medium containing 2.0 mg l KIN and 1