Tomographic Interference Microscopy of Living Cells

Abstract

Research on the internal structure of living cells has great interest for biology. For an optically transparent cell (phase sample) this problem can be solved only by tomographic methods. The methods of projection acquisi- tion and tomogram reconstruction are ana- lyzed. The tomographic interference microscopy for 3D refractive index spatial dis- tribution measurements has been proposed. The experimental setup is based on a Linnik interference microscope. Experimental work on lymphocyte reconstruction is described here.