Effect of Medium Composition on Xylanase Production by Bacillus subtilis using Various Agricultural Wastes

Abstract

In the present study a strain of Bacillus subtilis-BS05 was grown in submerged fermentation of agricultural wastes for xylanolytic activity. Various agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, soybean meal, corn cobs and wheat bran were evaluated for xylanolytic acitivity using different media (M-I, M-II, M-III & M-IV). Among all these tested substrates maximum xylanolytic activity was observed in sugarcane bagasse (439.5±2.84 IU) using medium constituents IV (M-IV) with agitation speed of 140rpm for 48h of fermentation period at 37°C. In static conditions maximum xylanase enzyme activity was recorded with rice husk (408.9±2.9 IU) at 37°C using media M-IV for 72h of fermentation period.