Coconut Water and Peptone Improve Seed Germination and Protocorm Like Body Formation of Hybrid Phalaenopsis

Abstract

Phalaenopsis Protocorm like body Coconut water Peptone Media culture Phalaenopsis is one of the most popular orchids in the market and it is easy to flowering. Orchid seed is rather difficult to germination rather than other angiospermic seeds, because of lack endosperm, radical and leaf rudiments. Seed germination and protocorm like body (PBL) formation of Phalaenopsis hybrid ‘Manchester’ was performed. This study was conducted to determine the effects of coconut water (CW), peptone and optimal media culture. Seeds from sterilized capsules cultured on five medium (modified FAST, modified KC, modified MS, modified ½ MS and modified VW) containing 0 and 15% (v/v) and 0 and 2 gl -1 peptone. The highest percentage of survival percentage on modified KC 2 gl -1 peptone without CW was observed. The highest germination percentage was observed in 15% (v/v), 2 gl -1 peptone and modified VW medium. This show no significant difference with modified FAST medium containing 15% (v/v) and 2 gl -1 peptone, modified KC medium containing 15% (v/v) without peptone and modified FAST medium containing 2 gl -1 peptone without CW and the highest PBL number formation was in modified ½ MS medium with peptone and CW with mean value 3.5. Overall, this study showed that Phalaenopsis seeds cultured on a ½ MS medium containing CW and peptone can be used for clone propagation.