PROTECTIVE EFFECT OF CHLOROGENIC ACID AGAINST DIABETIC NEPHROPATHY IN HIGH FAT DIET/STREPTOZOTOCIN INDUCED TYPE-2 DIABETIC RATS.

Abstract

Objective: Diabetic nephropathy (DN) is one the leading causes of death in diabetic patients. Treatment of DN is one of the major approaches towards decelerating the diabetes related complications. The present study was designed to investigate if chlorogenic acid (CGA) isolated from Coffea canephora could improve the renal function in Diabetic nephropathy (DN) by the virtue of its hypoglycemic and antioxidant activities. Methods: Male Wistar rats were randomly divided into four equal groups including normal healthy rats, diabetic untreated rats and two groups of diabetic rats treated with chlorogenic acid. Type-2 diabetes was induced by feeding the rats with high fat diet initially for 6 weeks followed by single intraperitoneal injection of streptozotocin (STZ) (35 mg/kg BW). CGA was supplied to the rats orally at two doses, 10 mg/kg BW/day and 20 mg/kg BW/day respectively over a period of 10 weeks post-induction of diabetes. After 10 weeks of STZ injection and CGA treatment, 24 h urine was collected and animals were sacrificed. Fasting blood glucose, glycosylated hemoglobin, biochemical markers of renal function and oxidative stress were evaluated in serum, urine and kidney tissue. Also the biochemical findings were matched with histopathological verification. Results: The results showed that treatment with CGA-10 mg/kg BW/day as well as CGA-20 mg/kg BW/day significantly improved the glycaemic status and renal function in Type-2 diabetic rats but CGA-20 mg/kg BW/day afforded greater efficiency as compared to CGA-10 mg/kg BW/day. Conclusion: The present investigation suggests that chlorogenic acid can be used as an alternative treatment against DN.