Characterization of Bacterial Pathogens Isolated from Calf Diarrhoea in Panchagarh District of Bangladesh

Abstract

The research work was conducted to isolate, identify and characterize the bacterial pathogens causing calf diarrhea in Panchagarh district of Bangladesh. The study was conducted on 114 faecal samples collected directly from the rectum of diarrhoeic calves and brought to the Microbiology laboratory of the Department of Microbiology, Hajee Mohammad Danesh Science and Technology Universiy, Dinajpur for bacteriological examination. Isolation and identification of the microorganisms were confirmed on the basis of their morphology, staining, cultural and biochemical tests. Furthermore, the isolated bacteria were characterized by antimicrobial susceptibility testing. A t ot al of 1 14 faecal sampl es were exami n ed for th e i sol ati on of bact eri a of whi ch 44 (38.6%) samples were positive for E. coli , 25 (21.9%) samples were positive for Salmonella spp, 15 (13.2%) samples were positive for Staphylococcus, 18(15.8%) samples were accounted for mixed infection and 12 (10.5%) samples were negative for bacteria. The antibiogram study revealed that most of the E. coli, Salmonella spp. and Staphylococcus spp. were resistant to penicillin, ampicillin, amoxycillin and bacitracin. However, most of the E. coli, Salmonella spp. and Staphylococcus spp. were susceptible to azithromycin, gentamicin and ciprofloxacin. The findings of the present study indicate that the use of azithromycin, gentamicin and ciprofloxacin may have the preference to be choice in clinical control of Salmonella, E. coli, and Staphylococcus causing calf diarrhoea in Panchagarh district of Bangladesh.