Cell Culture: History, Development and Prospects

Abstract

A B S T R A C T Since its inception, the animal cell culture in the twentieth century is related to its usefulness in research. Early methods such as Harrison culture of the inverted drop, Carrel's and Lindbergh's innovations made with the introduction of the use of the infusion pump, the culture of HeLa cells and vaccine design using animal cell cultures are approach which revolutioned the implementation and study of the cell culture. One of the important steps in this technique is the selection of the culture media, which provides the physical and chemical conditions close to those occurring in the natural environment for cell growth, which are crucial for the adhesion, proliferation and cell survival in vitro. In this review, the essential aspects that define this technique are shown, offering a historical overview of the most relevant events and the current prospect in the development of the cell culture, that have been enabled the progress in both, basic and applied research.