Segregation and Linkage of Several Genes in Cucumber

Abstract

Gene linkage was investigated in 11 families using 18 genes in cucumber ( Cucumis sativus L.). The genes studied were B (black spine), B-3 (Black spine-3), B-4 (Black spine-4), bi (bitterfree cotyledons), Bt (bitter fruit), Bt-2 (bitter fruit-2), D (dull fruit skin), df (delayed flowering), de (determinate habit), F (female sex expression), gl (glabrous foliage), lh (long hypocotyl), ns (numerous spines), pm-h [powdery mildew ( Sphaerotheca fuliginea Schlecht.:Fr.) resistance expressed on the hypocotyl], ss (small spines), Tu (tuberculate fruit), u (uniform immature fruit color), and w (white immature fruit color). A major objective of this study was to measure linkages of genes for fruit bitterness ( Bt and Bt-2 ), and spine color ( B-3 and B-4 ) relative to previously studied loci: B , bi , D , de , df , F , gl , lh , ns , pm-h , ss , Tu , u , and w . The F 2 progeny of LJ 90430 × PI 173889 segregated 13 bitter fruit: 3 nonbitter fruit, indicating that different genes are controlling fruit bitterness in these lines. Bt-2 is proposed as the gene controlling bitterness of fruit in LJ 90430. It is a separate locus from Bt , that causes bitter fruit in PI 173889. Several new gene linkages were found: bi — Bt , ( Bt-2 )— de , D —( Bt-2 ), D — ns , gl — F , ss —( Bt-2 ), Tu —( Bt-2 ), and u —( Bt-2 ). The Bt gene appears to be linked to bi and may be located on linkage group I. Bt-2 appears to be linked with several genes that could connect linkage groups I and IV. Bt-2 was linked to u , Tu , D , and ss, that are all on linkage group IV. Bt-2 was also found to be linked loosely to de , that is on linkage group I. No linkages were found between B-3 and B-4 and the genes evaluated in this study. Weak linkages (>25 cM) between several gene combinations [( Bt-2 )- de , de — ns , de — ss , de — Tu , de — u , ns — F , and ss — F ] provided more evidence that linkage group I and IV may be linked. Due to the weak linkages, more information needs to be obtained using larger populations and more markers to confirm these findings.