Rapid Micropropagation and Callus Induction of Catharanthus roseus in Vitro Using Different Explants

Abstract

Abstract: The effect of plant growth regulators on callus induction using different explants, to regenerate shoot/root from different explants as well as from calli in cultures of Catharanthus roseus (L.) G. Don was studied. Total of nineteen shoot regeneration media were used, out of these nineteen media three were further used to see the regeneration response and for root regeneration four media were tested supplemented with different concentration of IBA. Based on the results of this study, earliest and maximum (99%) callus induction th response was observed on 10 day of inoculation from hypocotyl explants under dark conditions on MS medium supplemented with BAP (1.0 mg/l) + NAA (1.0 mg/l). For shoot proliferation, MS basal medium 1 1 supplemented with BAP (1.5 mg l) + NAA (1.0 mg l) was the best followed by MS medium fortified with 1 1 1 BAP (3.0 mgl) + NAA (4.0 mgl) while half strength MS medium supplemented with IBA (2.5 mgl) + NAA 1 (0.5 mgl) gave best rooting response with quality roots. Besides, maximum shoot regeneration, response was observed from hypocotyl calli both under light and dark conditions on media supplemented with BAP (1.5 1 1 mgl) + NAA (1.0 mgl). Key words:6-Benzyladenine (BAP) Catharanthus roseus (L.) Indole butyric acid (IBA) Naphthalene acetic acid (NAA) Plant regeneration