Hybrid Organic-Inorganic Particle Technology: Breaking Through Traditional Barriers of HPLC Separations

Abstract

Hybrid organic‐inorganic particles combine the best properties of silica — high efficiency and excellent mechanical strength — with the best properties of organic polymers — a wide pH stability range and reduced silanol effects. In this article, the authors describe reversed-phase HPLC packings based on hybrid particles and demonstrate the packing’s retention and selectivity characteristics. They also show that these media have long lifetimes at elevated temperatures under conditions at which conventional bonded silica fails rapidly. The benefits of operating at elevated temperatures are increased efficiency and reduced back pressure. When combined with short columns containing 2.5- mm hybrid particles, these conditions enable fast, high-resolution gradient separations, which are useful for high-throughput analyses.