Molecular Characterization of Trichoderma viride and Trichoderma harzianum Isolated from Soils of North Bengal Based on rDNA Markers and Analysis of Their PCR-RAPD Profiles

Abstract

Nineteen isolates of Trichoderma viride and Trichoderma harzianum obtained from rhizosphere soil of plantation crops, forest soil and agricultural fields of North Bengal region were studied using RAPD and ITS-PCR. The genetic relatedness among eleven isolates of T. viride and eight isolates of T. harzianum were analyzed with six random primers. RAPD profiles showed genetic diversity among the isolates with the formation of eight clusters. Analysis of dendrogram revealed that similarity coefficient ranged from 0.67 to 0.95. ITS-PCR of rDNA region with ITS1 and ITS4 primers produced 600bp products in all isolates. This result indicated the identification patterns of Trichoderma isolates.