Phyto-chemical Screening and Antimicrobial Activities of Dhaman grass and Indian Ginseng

Abstract

Crude extracts of different parts (root, stem, leaf and seed) of Cenchrus ciliaris (CAZRI-358) and (Unripen fruit, ripen fruit and calyx) of Withania somnifera (RUBL-20668) were successively extracted with polar to non polar solvents using soxhlet assembly. The extracts were then screened for their Biological activity in-vitro against one gram positive bacteria (Staphylococcus aureus), two gram negative bacteria (Escherichia coli and Raoultella planticola) and one yeast (Candida albicans) by disc diffusion assay. Serial dilution method was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal/ fungicidal concentration (MBC/MFC). Water extract of Unripen fruit of W. somnifera showed highest antibacterial activity (IZ-13.33±0.24 mm, AI- 0.833) and highest antifungal activity (IZ-18.67±0.22 mm, AI- 2.667) against E. coli and C. albicans respectively as well as chloroform extract of stem (IZ-12.50±0.64 mm, AI- 1.042) and seeds (IZ-10.67±0.23 mm, AI- 0.821) of C. ciliaris against C. albicans and S. aureus.