An effective method for high frequency Multiple shoots regeneration and callus induction of Bacopa monnieri (L.) Pennel.:An important medicinal plant

Abstract

This review highlights the recent development and achievements made for the multiple shoots regeneration and callus induction of Bacopa monnieri. Shootlets were regenerated from nodal explants of stem through auxiliary shoot proliferation. The induction of multiple shoots from nodal segments were highest in MS medium supplemented with 0.5 mg/l BAP + 2.0 mg/l Kn and 0.5 mg/l Kn+1.0 mg/l BAP. For rooting different concentration of IBA were used and highest rooting was recorded on MS medium supplemented with 2.0 mg/l IBA. The rooted Plantlets were hardened initially in culture room conditions and then transferred to misthouse. Leaf petiole explants were used for the purpose of callus induction. Best growth was observed in MS medium supplemented with 0.25 mg/l 2, 4-D+ 0.5 mg/l Kn and 0.25 mg/l 2,4-D+ 0.1mg/l BAP.