Prevalence of Multidrug Resistant Bacteria Causing Late-Onset Neonatal Sepsis

Abstract

ISSN: 2319-7706 Volume 4 Number 5 (2015) pp. 172-190 http://www.ijcmas.com Neonatal sepsis is a leading cause of neonatal mortality in developing countries. Identification of the etiological agents of neonatal sepsis is essential for effective treatment. Out of 106 microbial isolates recovered from blood cultures of late onset neonatal sepsis (LONS) patients, only 70 (66 %) isolates were Gram-positive bacteria, 31 (29.2%) isolates were Gram-negative bacteria and 5 (4.7%) isolates were Candida sp. Coagulase negative staphylococci (CONS) was the most common causative LONS, which reached to 43(40.6%) of total isolates, followed by Micrococcus, Enterobacter, Coagulase positive staphylococci (COPS),Candida, Shigella, E. coli, Bacillus, Citrobacter and Klebsiella, which reached to 13(12.3%), 11(10.4%), 10(9.4%), 7(6.6%), 5(4.7%), 5(4.7%), 4(3.8%),4(3.8%) and 4(3.8%), respectively. CONS strains appeared more resistance to various test antibiotics compared to COPS. In addition, both of Staphylococcus and Enterobacterial isolates appeared high degree of resistance to penicillins and cephalosporins antibiotics, but their sensitivity to other tested antibiotics are different. In addition, about 50% of Staphylococcus isolates were resistant to aminoglycosides, IPM, glycopeptides and linezolid antibiotics, while 50% Enterobacterial isolates were resistant to glycopeptides, aminoglycosides, monobactam and tetracycline. The most resistant strains in the present study are Staphylococcus NBS-35, Staphylococcus NBS-98 and Enterobacter NBS-40which identified based on 16S rRNA gene sequence as strains of Staphylococcus epidermidis, Staphylococcus aureus subsp. aureus and Enterobacter cloacae, respectively. K ey wo rd s Neonatal sepsis, resistant, antibiotics, Staphylococcus,