RADICAL SCAVENGING ACTIVITIES AND NATURAL INDICATOR ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF Rosa Damascena

Abstract

Rosa damascena mill L. commonly known as rose having several pharmacological properties including anti-HIV, antibacterial, antioxidant, antitussive, hypnotic, anti-diabetic and relaxant effect on tracheal chains have been reported for this plant. The present works were carried out to evaluated antioxidant activity of Aqueous and ethanolic extract of rose flower petals. The antioxidant activities were measured using free radical scavenging activity with DPPH and reducing power assay. Being both dietary and biologically active compounds, flavonoids have attracted much attention of investigators as potent species capable of affecting various biological processes in living organisms. They are colour impart polyphenol. The total flavonoids contents of aqueous extract & ethanolic extracts of R.damascena flower petals were found to be12.73% and 32 % respectively. The HPLC analysis of ethanolic extract revealed presence of quarcetin having RT 2.33 min. Further, an attempt had been made to evaluated the used of above extract as herbal acid-base indicator in acid base titration. Natural pigments in plants are highly colour substances and may show colours changes with variation of pH. This attempt had been made to investigate the indicator activity of these extracts and to replace the synthetic indicators.