The Effects of Glyphosate, Auxin, and Cytokinin Combinations on in Vitro Development of Cranberry Node Explants

Abstract

Abstract Axillary shoot proliferation from cranberry ( Vaccinium macrocarpon Ait.) nodes in vitro was correlated positively with glyphosate [(N-(phosphonomethyl) glycine,(Gly)] and isopentenyladenine (2iP) concentrations. Maximum proliferation was caused by 3.7 × 10 −3 m Gly or 1.5 × 10 −4 m 2iP. Shoot proliferation was reduced when combined at levels above 1.9 × 10 −3 m Gly and 7.4 × 10 −5 m 2iP. Gly and 2iP reduced shoot and root dry weight. Indoleacetic acid (IAA) did not significantly affect shoot proliferation or shoot and root growth at concentrations of 5.7 × 10 −6 m and 5.7 × 10 −5 m . Gly induced basal swelling of shoots and reduced leaf size. These characteristic Gly effects were not altered by 2iP or IAA. Gly may be a useful addendum for in vitro shoot proliferation and a tool for the investigation of meristem initiation and development.