Effect of formaldehyde and binary ethyleneimine (BEI) on the integrity of foot and mouth disease virus capsid

Abstract

Summary: Formaldehyde treatment of foot and mouth disease (FMD) virus caused a statistically significant (p < 0.05) reduction in the concentration of 140S particles (26-72%) for 5 of the 6 South American vaccine virus strains tested. Formaldehyde also reduced the stability of the virus; after storage at 4°C for 6 months, there was a decay of 33-85% in the concentration of 140S particles. In contrast, samples treated with BEI did not undergo a significant reduction in 140S immediately after inactivation or after storage. The efficiency of infectivity inactivation by BEI was influenced by its concentration and temperature. No significant reduction in 140S was observed at 36°C in 24 hours.