PHENYLETHANOID GLYCOSIDES FROM BARLERIA CRISTATA L. CALLUS CULTURES

Abstract

Three phenylethanoid glycosides viz; -[(3′,4′-dihydroxyphenyl)-ethyl]-(4′َ′-O-caffeoyl)--D-glucoside (desrhamnosylacteoside) (1),  -[(3′,4′-dihydroxyphenyl)-ethyl]-(3′′-O-Lrhamnosyl)-(4′′-O-caffeoyl)- -D-glucoside (acteoside) (2) and  -[(3′,4′-dihydroxyphenyl)-ethyl]-(3′′,6′′-O-L-dirhamnosyl)-(4′′-O-caffeoyl)- -D-glucoside (poliumoside) (3) were isolatedand identified from the callus cultures of Barleria cristata L.The structures of the isolatedcompounds were established by spectroscopic evidence (UV, 1D and 2D-NMR and ESIMS),further confirmation has been done by comparison with authentic samples. The amount ofcompounds 1-3 were determined in the callus culture using HPLC.