PTC Micropropagation of Gloriosa superba L. Through High Frequency Shoot Proliferation

Abstract

Ninety two per cent of the cultures of apical and axillary buds of young sprouts from naturally grown Gloriosa superba plants regenerated four shoots per culture in MS basal medium fortified with 1.5 mg/l BA + 0.5 mg/l NAA. Repeated subcultures in the same medium resulted in rapid shoot multiplication with eight shoots per culture. Addition of 15% (v/v) coconut water (CW) and 2 g/l activated charcoal increased the number of shoots up to 15 per culture. In vitro raised shoots rooted on half strength MS with 1.0 mg/l IBA + 0.5 mg/l IAA added as a supplement. Plants in the rooting medium were kept at normal room temperature for seven days before transplanting in pots. Thereafter they were reared for three weeks. The survival rate of regenerants was found to be 85 90%.