Structure of the Monosialyl Oligosaccharides Derived from Salivary Gland Mucin Glycoproteins of the Chinese Swiftlet (Genus Collocalia)

Abstract

Abstracts of the XZIth International Carbohydrate Symposium, Utrecht, The Netherlands, p. 435, Vonk, Zeist. Portions of this paper (including Experimental Procedures, Tables I and 11, Figs. 1-8, and Refs. Sl-S7) are presented in miniprint at the end of this paper. Miniprint is easily read with the aid of a standard magnifying glass. Full size photocopies are available from the Journal of Biological Chemistry, 9650 Rockville Pike, Bethesda, MD 20814. Request Document No. 86 1050, cite the authors, and include a check or money order for $9.20 per set of photocopies. Full size photocopies are also included in the microfilm edition of the Journal that is available from Waverly Press. m/z 668,636 in the E1 spectrum reveal the composition of second branch to be Gal-Gal-GlcNAc; the sequence of latter is established from the presence of fragments mlz 1086 (M' minus Gal-Gal), m/z 1290 (M' minus Gal) and the absence of an ion pair at m/z 464, 432 (terminal Gal-GlcNAc, compare M6, see below). The intense ion mlz 182 is a further indication for the 1-4 linkage at the GlcNAc residue. In the 500-MHz 'H NMR spectrum of M5 (Fig. 6a), the GalNAc-ol H-2 and H-5 resonances are observed at