Prevalence of TEM, SHV and CTX-M genes in Escherichia coli and Klebsiella spp Urinary Isolates from Sudan with confirmed ESBL phenotype

Abstract

The aim of the present study was to identify some of the genes, namely: CTX-M, SHV, and TEM, responsible for extended-spectrum beta-lactamase (ESBL) phenomenon among Escherichia coli and Klebsiella spp isolated from Sudanese patients infected with urinary tract infection (UTI). Two hundred and eighteen Gram negative urinary isolates were collected at different hospitals in Khartoum State. Identification of the isolates was done by using conventional biochemical methods, and microbact 2000 24E system from Oxoid. ESBLs were screened according to CLSI guidelines. ESBLs Positive strains were tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, SHV and TEM genes. Out of 218 Escherichia coli and Klebsiella spp, ESBL was demonstrated in 130 (59.6%) of the isolates. The presences of CTX-M, SHV and TEM genes was confirmed in 68 (52.3%) of the isolates. The ESBL genes were detected in 19 Klebsiella spp and in 49 of Escherichia coli isolates. The communist frequent ESBL gene was CTX-M which was 48 and was observed in 35 and 13 of Escherichia coli and Klebsiella spp., respectively. The frequency of TEM gene was 38 and observed in 27 of Escherichia coli, and 11 of Klebsiella spp isolates. The frequency of SHV gene was 15 and observed in 3 and 12 of Escherichia coli and Klebsiella spp, respectively. It was concluded that all these genes were found to be carried by K. pneumoniae and Escherichia coli species. ESBL found to be higher in Sudan in comparison to other countries. Among urinary isolates the communist prevalence ESBL gene was CTX-M gene followed by TEM while the least one was SHV gene. (Omar B Ahmed, Alfadel O Omar, Atif H Asghar and Mogahid M Elhassan. Prevalence of TEM, SHV and CTX- M genes in Escherichia coli and Klebsiella spp Urinary Isolates from Sudan with confirmed ESBL phenotype.